Guanosine production and purine nucleotide biosynthetic enzymes in guanosine-producing mutants of Bacillus subtilis.
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منابع مشابه
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
The genes encoding the enzymes of IMP biosynthesis in Bacillus subtilis constitute the pur operon, whereas the genes encoding GMP biosynthetic enzymes, guaA (GMP synthetase) and guaB (IMP dehydrogenase), and the purA gene encoding adenylosuccinate (sAMP) synthetase all occur as single units. The purB gene encodes an enzyme involved in both IMP and AMP biosynthesis and is located in the pur oper...
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Demain, A. L. (Merck Sharp & Dohme Research Laboratories, Rahway, N.J.), I. M. Miller, and D. Hendlin. Production of extracellular guanosine-5'-monophosphate by Bacillus subtilis. J. Bacteriol. 88:991-995. 1964.-Wild-type Bacillus subtilis colonies were found to feed purineless mutants. A strain with high feeding capacity was selected for study, with a guanineless mutant of B. subtilis used as ...
متن کاملGuanosine triphosphatase stimulation of oncogenic Ras mutants.
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at codons 12 or 61, more rarely 13. Impaired GTPase activity, even in the presence of GTPase activating proteins, has been found to be the biochemical reason behind the oncogenicity of most Gly12/Gln61 ...
متن کاملA possible role of purine nucleotide pyrophosphorylases in the regulation of purine uptake by Bacillus subtilis.
The uptake of radioactive purines in resting cell suspensions of Bacillus subtilis has been studied. Initial rates of uptake obeyed saturation kinetics (e.g. apparent Km adenine, 0.6 PM). Subsequent uptake proceeded in steplike increments. Further studies were aimed at delineating the control mechanism for this uptake. Purines were highly concentrated with respect to the medium, but the intrace...
متن کاملCharacterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine
BACKGROUND Genome streamlining has emerged as an effective strategy to boost the production efficiency of bio-based products. Many efforts have been made to construct desirable chassis cells by reducing the genome size of microbes. It has been reported that the genome-reduced Bacillus subtilis strain MBG874 showed clear advantages for the production of several heterologous enzymes including alk...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1979
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.43.1317